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2015-05-18T01:56:19+05:30
In gram -ve bacteria cell wall is mainly consists of glycolipid . so when alcohol is used to wash the bacteria the lipid in the cellwall gets solubilised in the alcohol . so it fails to hold the gram stain. but in gram +ve bacteria cell wall is mainly consists of peptidoglycan, it can hold the stainĀ 
Thus alcohol is used to differentiate the gram +ve & gram -ve bacteria .

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