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Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome usingbiotechnology. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest usingmolecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or "knocked out", using a nucleaseGene targeting is a different technique that useshomologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.
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Genetic engineering is the technique in which the genetic constitution of an organism(bacterium) is altered by introducing new genes into its chromosomes.

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