Free help with homework

Why join Brainly?

  • ask questions about your assignment
  • get answers with explanations
  • find similar questions

Rearrange the following in the correct sequence to accomplish a biotechnological reaction: a...in vitro synthesis of dna of interest b...chemically

synthesized oligonucleotides c...enzyme dna polymerase d...complementary region of dna e...genomic dna template f....nucleotides g...primers h...thermostable dna polymerase i...denaturation of dna plsss reply fast...urgent


The sequence is as follows:
 first is denaturation in which the temperature is raised so that the double stranded DNA gets separated.
the second step is annealing where temperature is decreased  so that primers , chemically synthesised oligonucleotides can to both the strands and allows them to base pair with the complimentary strand pairs.
the third step is elongation where taq polymerase enzyme is added to the reaction that helps in the elongation of the nascent strand.this step also includes addition of ntp's

0 0 0
The Brain
  • The Brain
  • Helper
Not sure about the answer?
Learn more with Brainly!
Having trouble with your homework?
Get free help!
  • 80% of questions are answered in under 10 minutes
  • Answers come with explanations, so that you can learn
  • Answer quality is ensured by our experts