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- stain red or pink, they don't retain the Gram stain when washed with absolute    alcohol and acetone.
- it is single layered
 -outer membrane present
- LPS high
- lipid content high
- more resistance to antibiotic

Retain crystal violet dye and stain dark violet or purple, they remain coloured blue or purple with gram stain when washed with absolute alcohol and water.
- multilayered
- outer membrane absent
- LPH none
- lipid content low

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